DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island

Heilers J, Reiners J, Heller E, Golzer A, Smits S, van der Does C, Nucleic Acids Research (2019) DOI

SASDEF9 – TraI of Neisseria gonorrhoeae

MWI(0) 69 kDa
MWexpected 91 kDa
VPorod 293 nm3
log I(s) 6.73×101 6.73×100 6.73×10-1 6.73×10-2
TraI small angle scattering data  s, nm-1
ln I(s)
TraI Guinier plot ln 6.74×101 Rg: 7.3 nm 0 (7.3 nm)-2 s2
TraI Kratky plot 1.104 0 3 sRg
TraI pair distance distribution function Rg: 8.2 nm 0 Dmax: 31.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
TraI GASBOR model

Synchrotron SAXS data from solutions of TraI from Neisseria gonorrhoeae in 50 mM TRIS-HCl, pH 8, 100 mM NaCl, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Possible aggregates were removed using a Superdex 200 10/300 (GE Healthcare) size-exclusion chromatography (SEC) column pre-equilibrated with buffer at a flow rate of 0.5 mL/min at the EMBL-Lab outstation (Grenoble). All measurements were performed at 4°C. Sample concentration range was 0.7 to 10 mg/mL. For online-SEC one frame was collected every two seconds and for static measurements ten frames of data were collected with total exposure time of one second. By comparison of these frames, the possibility of radiation damage during the measurement was excluded. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. All used programs for data processing were part of the ATSAS Software package (Version 2.8.4).

TraI (TraI)
Mol. type   Protein
Organism   Neisseria gonorrhoeae
Olig. state   Monomer
Mon. MW   91.2 kDa
UniProt   Q5EPC7 (42-850)
Sequence   FASTA