Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Yadav DK, Zigáčková D, Zlobina M, Klumpler T Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ, Nucleic Acids Res (2019) Europe PMC

SASDF39 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1

ADP-ribosylation factor1 - short
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4
MWexperimental 29 kDa
MWexpected 31 kDa
VPorod 39 nm3
log I(s) 1.90×10-1 1.90×10-2 1.90×10-3 1.90×10-4
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 small angle scattering data  s, nm-1
ln I(s)
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 Guinier plot ln 1.90×10-1 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 Kratky plot 1.104 0 √3 sRg
p(r)
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 OTHER model
SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 in 50 mM sodium phosphate buffer, 300 mM NaCl, 500 mM imidazole, pH 8 were collected using a Rigaku BioSAXS-1000 instrument (CEITEC; Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 35°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ADP-ribosylation factor1 - short (ARF1 RNA - short)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   11.0 kDa
Sequence   FASTA
 
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 (STAU1-RBD34)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.7 kDa
 
UniProt   O95793 (183-355)
Sequence   FASTA