Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Yadav DK, Zigáčková D, Zlobina M, Klumpler T Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ, Nucleic Acids Res (2019) Europe PMC

SASDF49 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4
ADP-ribosylation factor1 - long
MWexperimental 34 kDa
MWexpected 36 kDa
VPorod 53 nm3
log I(s) 1.02×10-2 1.02×10-3 1.02×10-4 1.02×10-5
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long Guinier plot ln 1.02×10-2 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long pair distance distribution function Rg: 2.5 nm 0 Dmax: 7.0 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long in 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH 6.8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1025 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 15°C. One 3 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 (STAU1-RBD34)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.7 kDa
 
UniProt   O95793 (183-355)
Sequence   FASTA
 
ADP-ribosylation factor1 - long (ARF1 RNA - long)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   16.1 kDa
Sequence   FASTA