Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.

Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E, J Biol Chem (2019) Europe PMC

SASDF57 – Complex of CBP KIX domain with BMAL1 (G517-L625) including C-terminal transactivation domain (TAD) (Mus musculus)

Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP)
MWexperimental 21 kDa
MWexpected 22 kDa
log I(s) 5.68×103 5.68×102 5.68×101 5.68×100
Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) small angle scattering data  s, nm-1
ln I(s)
Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Guinier plot ln 5.68×103 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Kratky plot 1.104 0 3 sRg
p(r)
Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) pair distance distribution function Rg: 3.4 nm 0 Dmax: 13.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) DAMMIN model

Synchrotron SAXS data from solutions of Complex of CBP KIX domain with BMAL1 (G517-L625) including C-terminal transactivation domain (TAD) (Mus musculus) in 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.37 mg/ml was measured at 10.2°C. 30 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) (BMAL1-(517-625))
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   11.1 kDa
 
UniProt   Q9WTL8-2 (517-625)
Sequence   FASTA
 
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) (CBP KIX domain)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   10.5 kDa
 
UniProt   P45481 (586-672)
Sequence   FASTA