Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.

Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E, J Biol Chem (2019) Europe PMC

SASDF67 – Complex of CBP KIX domain with BMAL1 (G490-L625) including C-terminal transactivation domain (TAD) (Mus musculus)

Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP)
Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD)
MWexperimental 33 kDa
MWexpected 25 kDa
log I(s) 7.30×103 7.30×102 7.30×101 7.30×100
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) small angle scattering data  s, nm-1
ln I(s)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) Guinier plot ln 7.31×103 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) Kratky plot 1.104 0 3 sRg
p(r)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) pair distance distribution function Rg: 4.3 nm 0 Dmax: 17.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) DAMMIN model

Synchrotron SAXS data from solutions of Complex of CBP KIX domain with BMAL1 (G490-L625) including C-terminal transactivation domain (TAD) (Mus musculus) in 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.29 mg/ml was measured at 10.2°C. 30 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) (CBP KIX domain)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   10.5 kDa
 
UniProt   P45481 (586-672)
Sequence   FASTA
 
Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) (BMAL1-(490-625))
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   14.0 kDa
 
UniProt   Q9WTL8-2 (490-625)
Sequence   FASTA