data from solutions of
Insulin glargine (Toujeo®), oligomeric composition
Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH 4
EMBL P12 beam line
at the PETRA III storage ring
using a Pilatus 6M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 1.241 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 10.91 mg/ml was measured
0.045 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Here, Toujeo measured at 10.91 mg/ml is displayed (formulation concentration).
Measurements from 2 dilutions have been deposited in addition. Mixture analysis of the scattering profiles was performed with the program Oligomer. Data can be fit with a stable equilibrium of ~ 60 % hexamers (based on 1ev3.pdb) and ~ 40 % dimers. For higher concentration a strong influence of
interparticle interference is observed, so that data at low s (>0.05 nm-1) is omitted for the fit. The presence of 3% dodecamers (based on 3W80.pdb)
slightly improves the fit.