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17 hits found for Graewert

SASDF94 – Insulin glulisine (Apidra), oligomeric composition

Insulin glulisine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Insulin glulisine hexamer, 35 kDa protein
Buffer: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20), pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 20
The quaternary structure of insulin glargine and glulisine under formulation conditions Biophysical Chemistry :106226 (2019)
...Graewert M, Gao M, Heyse W, Jeffries C, Svergun D, Berchtold H
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDA28 – anti-TG2 antibody (679 14 E06)

anti-TG2 antibody (679 14 E06)  experimental SAS data
CRYSOL model
Sample: anti-TG2 antibody (679 14 E06) monomer, 48 kDa protein
Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17
Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease. J Biol Chem 290(35):21365-75 (2015)
...Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM
RgGuinier 2.5 nm
Dmax 8.1 nm
VolumePorod 58 nm3

SASDA38 – transglutaminase-2 (TGA2)

transglutaminase 2 experimental SAS data
CRYSOL model
Sample: transglutaminase 2 monomer, 79 kDa Homo sapiens protein
Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17
Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease. J Biol Chem 290(35):21365-75 (2015)
...Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 117 nm3

SASDA48 – transglutaminase2:anti-transglutaminase2 FAB1 antibody complex

anti-TG2 antibody (679 14 E06) transglutaminase 2 experimental SAS data
DAMMIN model
Sample: anti-TG2 antibody (679 14 E06) monomer, 48 kDa protein
transglutaminase 2 monomer, 79 kDa Homo sapiens protein
Buffer: 20 mM Tris 150mM NaCl 1mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 17
Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease. J Biol Chem 290(35):21365-75 (2015)
...Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 168 nm3

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23
SASBDB Standard Proteins
Melissa Graewert, Cy M Jeffries
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3

SASDF24 – Full-length X-chromosome linked inhibitor of apoptosis protein (XIAP)

E3 ubiquitin-protein ligase XIAP experimental SAS data
HADDOCK model
Sample: E3 ubiquitin-protein ligase XIAP dimer, 113 kDa Homo sapiens protein
Buffer: Xiap buffer, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 15
Conformational characterization of full-length X-chromosome-linked inhibitor of apoptosis protein (XIAP) through an integrated approach IUCrJ 6(5) (2019)
...Graewert M, Svergun D, Banci L
RgGuinier 3.9 nm
Dmax 12.8 nm
VolumePorod 207 nm3

SASDA97 – PlaB

PlaB experimental SAS data
DAMMIN model
Sample: PlaB tetramer, 220 kDa Legionella pneumophila protein
Buffer: 100 mM Tris 100 mM Nacl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 15
Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions. Sci Rep 5:10734 (2015)
Graewert MA, Franke D, Jeffries CM, Blanchet CE, Ruskule D, Kuhle K, Flieger A, Schäfer B, Tartsch B, Meijers R, Svergun DI
RgGuinier 4.0 nm
Dmax 10.5 nm
VolumePorod 270 nm3

SASDFA4 – Insulin glargine (Toujeo®), oligomeric composition

Insulin glargine (Toujeo®) experimental SAS data
PDB model
Sample: Insulin glargine (Toujeo®) hexamer, 36 kDa protein
Buffer: Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 5
The quaternary structure of insulin glargine and glulisine under formulation conditions Biophysical Chemistry :106226 (2019)
...Graewert M, Gao M, Heyse W, Jeffries C, Svergun D, Berchtold H
RgGuinier 1.8 nm
Dmax 6.2 nm

SASDFB4 – Insulin glargine (Lantus ®), oligomeric mixture

Insulin glargine (Lantus ®) experimental SAS data
Insulin glargine (Lantus ®) Kratky plot
Sample: Insulin glargine (Lantus ®) hexamer, 36 kDa protein
Buffer: Lantus Formulation (30 µg Zinc cloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 23
The quaternary structure of insulin glargine and glulisine under formulation conditions Biophysical Chemistry :106226 (2019)
...Graewert M, Gao M, Heyse W, Jeffries C, Svergun D, Berchtold H
RgGuinier 1.8 nm
Dmax 5.3 nm

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data
DAMMIN model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain ... protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
SASBDB Standard Proteins
Melissa Graewert, Cy M Jeffries
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 201 nm3

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB model
Sample: Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data
DAMMIN model
Sample: Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
SASBDB Standard Proteins
Melissa Graewert, Cy M Jeffries
RgGuinier 5.4 nm
Dmax 12.5 nm
VolumePorod 679 nm3

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data
DAMMIN model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
SASBDB Standard Proteins
Melissa Graewert, Cy M Jeffries
RgGuinier 1.8 nm
Dmax 5.1 nm
VolumePorod 37 nm3

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
SASBDB Standard Proteins
Melissa Graewert, Cy M Jeffries
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
SASBDB Standard Proteins
Melissa Graewert, Cy M Jeffries
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDBS2 – Recombinant Tn antigen-binding lectin from Vatairea macrocarpa

Recombinant Tn antigen-binding lectin experimental SAS data
CORAL model
Sample: Recombinant Tn antigen-binding lectin tetramer, 105 kDa Vatairea macrocarpa protein
Buffer: 100 mM sodium phosphate 150 mM NaCl 5% (v/v) glycerol, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 11
Structural characterization of a Vatairea macrocarpa lectin in complex with a tumor-associated antigen: A new tool for cancer research. Int J Biochem Cell Biol 72:27-39 (2016)
...Graewert MA, Pereira RI, Cunha RMS, Nascimento KS, Bezerra GA, Delatorre P, Djinovic-Carugo K, Nagano CS, Gruber K, Cavada BS
RgGuinier 3.2 nm
Dmax 9.5 nm
VolumePorod 168 nm3