Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization

Sharma M, Aswal V, Kumar V, Chidambaram R, IUCrJ 7(2) (2020) DOI

SASDFA7 – Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O

Lysinibacillus Mosquito-larvicidal receptor binding component
MWI(0) 62 kDa
MWexpected 53 kDa
log I(s) 4.50×10-2 4.50×10-3 4.50×10-4 4.50×10-5
Lysinibacillus Mosquito-larvicidal receptor binding component small angle scattering data  s, nm-1
ln I(s)
Lysinibacillus Mosquito-larvicidal receptor binding component Guinier plot ln 4.50×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Lysinibacillus Mosquito-larvicidal receptor binding component Kratky plot 1.104 0 3 sRg
p(r)
Lysinibacillus Mosquito-larvicidal receptor binding component pair distance distribution function Rg: 3.2 nm 0 Dmax: 9.9 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Lysinibacillus Mosquito-larvicidal receptor binding component PDB model

log I(s)
 s, nm-1
Lysinibacillus Mosquito-larvicidal receptor binding component DAMMIN model

SANS data from solutions of Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O in 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH 7.5 were collected using a Ingenuously built detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.52 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.30 mg/ml was measured at 25°C. One 43200 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Experimental Molecular weight Value estimated using I(0) values from GNOM analysis. Intensity has been normalized to protein concentration of 1 mg/ml. Actual sample used was 3.3 mg/ml.

Lysinibacillus Mosquito-larvicidal receptor binding component (BinB)
Mol. type   Protein
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   53.5 kDa
 
UniProt   B1HQ59
Sequence   FASTA
 
PDB code   5FOY