Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization

Sharma M, Aswal V, Kumar V, Chidambaram R
IUCrJ 7(2) (2020 Jan 25)

doi: 10.1107/S2052252519017159
Submitted to SASBDB: 2019 Jun 9
Published in SASBDB:

SASDF87 – Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O

Binary toxin receptor Cqm1 protein experimental SAS data
PDB model
Sample: Binary toxin receptor Cqm1 protein dimer, 129 kDa synthetic construct protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24
RgGuinier 2.8 nm
Dmax 9.7 nm

SASDF97 – Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O

Binary toxin receptor Cqm1 proteinDeuterated BinB component of mosquito-larvicidal Binary toxin experimental SAS data
PDB model
Sample: Binary toxin receptor Cqm1 protein monomer, 65 kDa synthetic construct protein
Deuterated BinB component of mosquito-larvicidal Binary toxin monomer, 56 kDa synthetic construct protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 23
RgGuinier 1.9 nm
Dmax 7.3 nm

SASDFA7 – Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O

Lysinibacillus Mosquito-larvicidal receptor binding component experimental SAS data
PDB model
Sample: Lysinibacillus Mosquito-larvicidal receptor binding component monomer, 53 kDa synthetic construct protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24
RgGuinier 3.3 nm
Dmax 9.9 nm