Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.

Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT, J Mol Biol (2019) Europe PMC

SASDFL6 – Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8

Adenine specific DNA methyltransferase (Mod)
MWexperimental 120 kDa
MWexpected 137 kDa
VPorod 143 nm3
log I(s) 3.55×101 3.55×100 3.55×10-1 3.55×10-2
Adenine specific DNA methyltransferase (Mod) small angle scattering data  s, nm-1
ln I(s)
Adenine specific DNA methyltransferase (Mod) Guinier plot ln 3.56×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Adenine specific DNA methyltransferase (Mod) Kratky plot 1.104 0 3 sRg
p(r)
Adenine specific DNA methyltransferase (Mod) pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Adenine specific DNA methyltransferase (Mod) PYMOL model

log I(s)
 s, nm-1
Adenine specific DNA methyltransferase (Mod) PYMOL model

Synchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8 in 25 mM Tris, 250 mM NaCl were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Adenine specific DNA methyltransferase (Mod)
Mol. type   Protein
Organism   Helicobacter pylori (strain ATCC 700392 / 26695)
Olig. state   Dimer
Mon. MW   68.3 kDa
 
UniProt   O25315 (1-598)
Sequence   FASTA