Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.

Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT
J Mol Biol (2019 Oct 16)
PMID: 31628946
doi: 10.1016/j.jmb.2019.10.001
Submitted to SASBDB: 2019 May 31
Published in SASBDB:

SASDFL6 – Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8

Adenine specific DNA methyltransferase (Mod) experimental SAS data
PYMOL model
Sample: Adenine specific DNA methyltransferase (Mod) dimer, 137 kDa Helicobacter pylori (strain ... protein
Buffer: 25 mM Tris, 250 mM NaCl, pH: 8
Experiment: SAXS data collected at ID14-3, ESRF on 2017 Jul 9
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 143 nm3

SASDFM6 – Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5

Adenine specific DNA methyltransferase (Mod) experimental SAS data
PYMOL model
Sample: Adenine specific DNA methyltransferase (Mod) tetramer, 273 kDa Helicobacter pylori (strain ... protein
Buffer: 25 mM citrate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 1
RgGuinier 5.0 nm
Dmax 19.1 nm
VolumePorod 316 nm3