Quaternary structure of α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) controls its activity.

Yang Y, Davis I, Matsui T, Rubalcava I, Liu A, J Biol Chem (2019) Europe PMC

SASDFN5 – Wild type 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, ACMSD tetramer, at pH 7.0

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase
MWexperimental 159 kDa
MWexpected 159 kDa
VPorod 195 nm3
log I(s) 5.18×101 5.18×100 5.18×10-1 5.18×10-2
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase small angle scattering data  s, nm-1
ln I(s)
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase Guinier plot ln 5.19×101 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase Kratky plot 1.104 0 3 sRg
p(r)
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase pair distance distribution function Rg: 4.8 nm 0 Dmax: 17.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase CORAL model

Synchrotron SAXS data from solutions of wild type ACMSD tetramer in 25 mM HEPES, 5 mM DTT, pH 7 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1088 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed at 25°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase (ACMSD)
Mol. type   Protein
Organism   Pseudomonas fluorescens
Olig. state   Tetramer
Mon. MW   39.7 kDa
 
UniProt   Q83V25 (1-334)
Sequence   FASTA