Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.

Sajko S, Grishkovskaya I, Kostan J, Graewert M Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K, PLoS One 15(12):e0242677 (2020) Europe PMC

SASDG97 – Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15

Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15
MWexperimental 74 kDa
MWexpected 78 kDa
VPorod 187 nm3
log I(s) 5.42×101 5.42×100 5.42×10-1 5.42×10-2
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 small angle scattering data  s, nm-1
ln I(s)
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 Guinier plot ln 5.42×101 Rg: 6.5 nm 0 (6.5 nm)-2 s2
(sRg)2I(s)/I(0)
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 Kratky plot 1.104 0 √3 sRg
p(r)
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 pair distance distribution function Rg: 7.3 nm 0 Dmax: 26 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 DAMMIF model
log I(s)
 s, nm-1
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15 in 20 mM Tris-HCl, 100 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 3.8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 90 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 (tbMORNdelta1)
Mol. type   Protein
Organism   Trypanosoma brucei
Olig. state   Dimer
Mon. MW   39.2 kDa
 
UniProt   Q587D3 (26-358)
Sequence   FASTA