| MWexperimental | 46 | kDa |
| MWexpected | 52 | kDa |
| VPorod | 56 | nm3 |
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log I(s)
2.82×103
2.82×102
2.82×101
2.82×100
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s, nm-1
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Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.
Sajko S, Grishkovskaya I, Kostan J, Graewert M Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K, PLoS One 15(12):e0242677 (2020) Europe PMCSASDGA7 – Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN (7-15)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15
in
20 mM Tris-HCl, 200 mM NaCl, 2% (w/v) glycerol, 0.5 mM DTT, pH 8.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 3.8 mg/ml was injected at a 0.50 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
60 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN |
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