Apolipoprotein E project

Lucas Kraft.

SASDGA8 – Apolipoprotein E3 bound to 330 µM Suramin

Apolipoprotein E3
Suramin
MWexperimental 164 kDa
MWexpected 140 kDa
log I(s) 5.40×10-1 5.40×10-2 5.40×10-3 5.40×10-4
Apolipoprotein E3 Suramin small angle scattering data  s, nm-1
ln I(s)
Apolipoprotein E3 Suramin Guinier plot ln 5.40×10-1 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
Apolipoprotein E3 Suramin Kratky plot 1.104 0 3 sRg
p(r)
Apolipoprotein E3 Suramin pair distance distribution function Rg: 5.4 nm 0 Dmax: 18.6 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Apolipoprotein E3 bound to 330 µM Suramin in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 28 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ApoE3 + 330 µM Suramin

Apolipoprotein E3 (ApoE3)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   34.7 kDa
 
UniProt   P02649 (19-317)
Sequence   FASTA
 
Suramin
Mol. type   Other
Olig. state   Monomer
Mon. MW   1.3 kDa
Chemical formula