Li de la Sierra-Gallay I,
Le Cam E,
Nucleic Acids Res
SASDGR5 – The Vibrio cholerae DciA/DnaB helicase complex in the presence of bound ATP (VcDnaB.DciA.ATP)
Synchrotron SAXS data from solutions of the DnaB helicase hexamer/DciA complex in 20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH 8.8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 84.00 μl sample at 27.8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 15°C. 700 successive 2.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The scattered intensities were displayed on an absolute scale (cm-1) using the scattering of water. Frames were examined individually using the US-SOMO HPLC module and 6 identical frames obtained at the beginning of the elution peak were averaged and further processed.
The complex is constituted of one hexamer of DnaB and three monomers of DciA.
DnaB helicase complexed with ATP
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)