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21 hits found for Durand

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P,...
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 229 nm3

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

Calmodulin-1 experimental SAS data
4Ca2+-calmodulin - Xenopus laevis Rg histogram
Sample: Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P,...
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 25 nm3

SASDCJ3 – Bovine Serum Albumin

Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P,...
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDEJ3 – Truncated neutophil cytosol factor 1, p47phox [1-342]

Neutophil cytosol factor 1 experimental SAS data
OTHER model
Sample: Neutophil cytosol factor 1 monomer, 40 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2009 Oct 16
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 58 nm3

SASDEK3 – The neutrophil cytosol factor 1 (p47phox) subunit of phagocyte NADPH oxidase

Neutrophil cytosol factor 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Neutrophil cytosol factor 1 monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2008 Apr 23
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 77 nm3

SASDEL3 – The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase

Neutrophil cytosol factor 2 experimental SAS data
The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase Rg histogram
Sample: Neutrophil cytosol factor 2 monomer, 61 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 8
Experiment: SAXS data collected at D24, LURE on 2003 Apr 9
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 113 nm3

SASDJ64 – Calcium bound Calmodulin

Calmodulin-1 experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
...Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 27 nm3

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
...Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDGM5 – Holo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
...Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 4.4 nm
Dmax 15.5 nm
VolumePorod 89 nm3

SASDGN5 – apo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
...Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 8.3 nm
Dmax 33.0 nm

SASDGP5 – Vibrio cholerae DciA

DciA experimental SAS data
DciA Kratky plot
Sample: DciA monomer, 18 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 26
Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase. Nucleic Acids Res (2021)
...Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL
RgGuinier 2.7 nm
Dmax 10.1 nm
VolumePorod 26 nm3

SASDGQ5 – Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP)

Replicative DNA helicase (DnaB) experimental SAS data
Replicative DNA helicase (DnaB) Kratky plot
Sample: Replicative DNA helicase (DnaB) hexamer, 317 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Oct 4
Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase. Nucleic Acids Res (2021)
...Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL
RgGuinier 4.6 nm
Dmax 13.0 nm
VolumePorod 585 nm3

SASDGR5 – The Vibrio cholerae DciA/DnaB helicase complex in the presence of bound ATP (VcDnaB.DciA.ATP)

DnaB helicase complexed with ATPDciA experimental SAS data
DnaB helicase complexed with ATP DciA Kratky plot
Sample: DnaB helicase complexed with ATP hexamer, 317 kDa Vibrio cholerae serotype … protein
DciA , 55 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Oct 4
Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase. Nucleic Acids Res (2021)
...Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL
RgGuinier 5.0 nm
Dmax 15.6 nm
VolumePorod 670 nm3

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data
BUNCH model
Sample: Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 175 nm3

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data
OTHER model
Sample: comcde , 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 11
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 122 nm3

SASDAC7 – Complex LytTR-comcde

comcdeResponse regulator experimental SAS data
SASREF model
Sample: comcde , 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 29 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jun 2
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 74 nm3

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data
EKC/KEOPS complex subunit GON7 Kratky plot
Sample: EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 26
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, ...
RgGuinier 3.1 nm
Dmax 12.5 nm
VolumePorod 46 nm3

SASDFL8 – GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3Probable tRNA N6-adenosine threonylcarbamoyltransferase experimental SAS data
OTHER model
Sample: EKC/KEOPS complex subunit GON7 monomer, 12 kDa Homo sapiens protein
EKC/KEOPS complex subunit LAGE3 monomer, 15 kDa Homo sapiens protein
Probable tRNA N6-adenosine threonylcarbamoyltransferase monomer, 36 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 May 12
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, ...
RgGuinier 3.1 nm
Dmax 11.5 nm
VolumePorod 91 nm3

SASDFM8 – GON7, the fifth subunit of human KEOPS, bound to the EKC/KEOPS complex subunit, LAGE3

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3 experimental SAS data
EKC/KEOPS complex subunit GON7 EKC/KEOPS complex subunit LAGE3 Kratky plot
Sample: EKC/KEOPS complex subunit GON7 dimer, 25 kDa Homo sapiens protein
EKC/KEOPS complex subunit LAGE3 dimer, 33 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 19
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, ...
RgGuinier 3.6 nm
Dmax 15.5 nm
VolumePorod 126 nm3

SASDCK9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data
ALLOSMOD model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
...Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.6 nm
Dmax 9.1 nm
VolumePorod 60 nm3

SASDCL9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data
BUNCH model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
...Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 78 nm3