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8 hits found for Durand

SASDEL3 – The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase

Neutrophil cytosol factor 2 experimental SAS data
The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase Rg histogram
Sample: Neutrophil cytosol factor 2 monomer, Homo sapiens protein
Buffer: 20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 8
Experiment: SAXS data collected at D24, LURE on 2003 Apr 9
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 113 nm3

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data
BUNCH model
Sample: Histidine protein kinase dimer, Streptococcus pneumoniae protein
Response regulator dimer, Streptococcus pneumoniae protein
Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 175 nm3

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data
OTHER model
Sample: comcde other, Streptococcus pneumoniae DNA
Response regulator dimer, Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 11
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 122 nm3

SASDAC7 – Complex LytTR-comcde

comcdeResponse regulator experimental SAS data
SASREF model
Sample: comcde other, Streptococcus pneumoniae DNA
Response regulator dimer, Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jun 2
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 74 nm3

SASDEK3 – The neutrophil cytosol factor 1 (p47phox) subunit of phagocyte NADPH oxidase

Neutrophil cytosol factor 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Neutrophil cytosol factor 1 monomer, Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2008 Apr 23
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 77 nm3

SASDEJ3 – Truncated neutophil cytosol factor 1, p47phox [1-342]

Neutophil cytosol factor 1 experimental SAS data
OTHER model
Sample: Neutophil cytosol factor 1 monomer, Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2009 Oct 16
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 58 nm3

SASDCK9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data
ALLOSMOD model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, Bordetella pertussis (strain ... protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
...Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.6 nm
Dmax 9.1 nm
VolumePorod 60 nm3

SASDCL9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data
BUNCH model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, Bordetella pertussis (strain ... protein
Calmodulin monomer, Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
...Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 78 nm3