Synchrotron SAXS data from solutions of truncated poly(rC)-binding protein 2 (PCBP2-KH1-KH2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) in 5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.10322 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100 μl sample was injected at a 0.40 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 15°C. 720 successive 5 second frames were collected through the SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection concentration = UNKNOWN
modified stem loop IV poliovirus IRES, nucleotides 278-398