data from solutions of
Braveheart Fragment 3
50 mM HEPES-KOH, 100 mM KCl, 6 mM MgCl2, pH 7.6
B21 beam line
at the Diamond Light Source storage ring
using a Pilatus 2M detector
at a sample-detector distance of 4 m and
at a wavelength of λ = 0.1 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 0.5 mg/ml was injected
onto a column
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
This fragment contains nucleotides 291 to 588 of Braveheart RNA and 3' structure cassette for primer extension after SHAPE and DMS probing (nt # 589-634). SEC-column type and SEC parameters (flow-rate, injection volume) = UNKNOWN; Experimental temperature = UNKNOWN
|Braveheart Fragment 3