Solution structure of ectodomains of the insulin receptor family: the ectodomain of the type 1 insulin-like growth factor receptor displays asymmetry of ligand binding accompanied by limited conformational change.

Whitten AE, Smith BJ, Menting JG, Margetts MB, McKern NM, Lovrecz GO, Adams TE, Richards K, Bentley JD, Trewhella J, Ward CW, Lawrence MC, J Mol Biol 394(5):878-92 (2009) Europe PMC

SASDHF2 – Insulin receptor ectodomains (IRΔβ)

Insulin receptor
MWI(0) 260 kDa
MWexpected 203 kDa
VPorod 385 nm3
log I(s) 4.04×10-1 4.04×10-2 4.04×10-3 4.04×10-4
Insulin receptor small angle scattering data  s, nm-1
ln I(s)
Insulin receptor Guinier plot ln 4.04×10-1 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
Insulin receptor Kratky plot 1.104 0 √3 sRg
p(r)
Insulin receptor pair distance distribution function Rg: 5.5 nm 0 Dmax: 17 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Insulin receptor BUNCH model
X-ray scattering data from the from the human insulin receptor in 30 mM Tris 140mM NaCl 0.02% w/v azide pH 7.5 were collected on a Bruker Nanostar instrument at the Bragg Institute (Australian Nuclear Science and Technology Organisation, Lucas Heights, Australia) using a VÅNTEC-2000 detector (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle; λ=0.15406 nm). Approximately 30 uL of a 2.0 mg/ml protein solution was loeaded into a quarz capilary mounted in a stainless steel holder. Two successive 1800 second frames were collected, and the buffer was collected in an analogous fashion. The data were normalized to the intensity of the transmitted beam, radially averaged, placed on an absolute scale against a water standard, and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from a rigid body model using BUNCH08.

Additional modelling information, including summaries for the related entry SASDHE2 (Type 1 insulin-like growth factor receptor ectodomains, IGF-1RΔβ), are provided in the full entry zip-archive.

Insulin receptor (IR)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   101.6 kDa
 
UniProt   P06213-1 (28-948)
Sequence   FASTA