The hTERT core promoter forms three parallel G-quadruplexes.

Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO, Nucleic Acids Res (2020) Europe PMC

SASDHM3 – Human Telomerase Reverse Transcriptase Core Promoter G-rich Region (hTERT_WT) DNA

Human Telomerase Reverse Transcriptase Core Promoter G-rich Region
MWexperimental 22 kDa
MWexpected 22 kDa
VPorod 28 nm3
log I(s) 3.59×10-4 3.59×10-5 3.59×10-6 3.59×10-7
Human Telomerase Reverse Transcriptase Core Promoter G-rich Region small angle scattering data  s, nm-1
ln I(s)
Human Telomerase Reverse Transcriptase Core Promoter G-rich Region Guinier plot ln 3.59×10-4 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Human Telomerase Reverse Transcriptase Core Promoter G-rich Region Kratky plot 1.104 0 √3 sRg
p(r)
Human Telomerase Reverse Transcriptase Core Promoter G-rich Region pair distance distribution function Rg: 2.3 nm 0 Dmax: 8.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Telomerase Reverse Transcriptase Core Promoter G-rich Region DAMMIF model
Synchrotron SAXS data from solutions of hTERT_WT in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 4 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Human Telomerase Reverse Transcriptase Core Promoter G-rich Region (TERT)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   21.7 kDa
Sequence   FASTA