RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression

Knittel V, Sadana P, Seekircher S, Stolle A, Körner B, Volk M, Jeffries C, Svergun D, Heroven A, Scrima A, Dersch P, Mecsas J, PLOS Pathogens 16(9):e1008552 (2020) DOI

SASDHP5 – Rov C - Regulator Of Virulence interconnected with the Csr system (hexameric transcriptional activator of type VI secretion gene expression)

Regulator Of Virulence interconnected with the Csr system
MWexperimental 175 kDa
MWexpected 175 kDa
VPorod 280 nm3
log I(s) 9.15×103 9.15×102 9.15×101 9.15×100
Regulator Of Virulence interconnected with the Csr system small angle scattering data  s, nm-1
ln I(s)
Regulator Of Virulence interconnected with the Csr system Guinier plot ln 9.16×103 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Regulator Of Virulence interconnected with the Csr system Kratky plot 1.104 0 3 sRg
p(r)
Regulator Of Virulence interconnected with the Csr system pair distance distribution function Rg: 4.1 nm 0 Dmax: 12.4 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Regulator Of Virulence interconnected with the Csr system CORAL model

log I(s)
 s, nm-1
Regulator Of Virulence interconnected with the Csr system GASBOR model

log I(s)
 s, nm-1
Regulator Of Virulence interconnected with the Csr system DAMMIN model

Synchrotron SAXS data from solutions of Rov C in 50 mM TRIS pH= 8, 500 mM NaCl, 5 mM DTT, 5 % v/v glycerol, were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.5 and 5 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

The experimental molecular weight (MW) was determined using multi angle light scattering coupled to refractive index measurements. The concentration-independent MW estimate from the SAXS data (Bayesian inference) is approximately 185 kDa in the credibility interval of 162-195 kDa. The SAXS data presented in this entry show merged data obtained from two sample concentrations (2.5 and 5.0 mg/ml). The low-angle data measured from the 2.5 mg/ml sample (0.073 < s < 0.63 nm-1) were manually scaled and merged with the high-angle data from the 5 mg/ml profile (0.23 < s < 2.6 nm-1). Additional SAXS data measured from samples through the a concentration series (5, 2.5, 1.25 and 0.625 mg/ml) are provided in the full entry zip archive. Additional GASBOR, DAMMIN and CORAL models, as well as their spatial alignments, are also made available in the archive.

Regulator Of Virulence interconnected with the Csr system (Rov C)
Mol. type   Protein
Organism   Yersinia pseudotuberculosis
Olig. state   Hexamer
Mon. MW   29.2 kDa
 
UniProt   A0A0H3B5N9 (1-247)
Sequence   FASTA
 
PDB code   6XZ5