Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst

Markova K, Chmelova K, Marques S, Carpentier P, Bednar D, Damborsky J, Marek M, Chemical Science 11(41):11162-11178 (2020) DOI

SASDHQ7 – Haloalkane dehalogenase variant DhaA115 - dimeric fraction

Haloalkane dehalogenase variant DhaA115 - dimeric fraction
MWexperimental 49 kDa
MWexpected 69 kDa
VPorod 78 nm3
log I(s) 1.68×100 1.68×10-1 1.68×10-2 1.68×10-3
Haloalkane dehalogenase variant DhaA115 - dimeric fraction small angle scattering data  s, nm-1
ln I(s)
Haloalkane dehalogenase variant DhaA115 - dimeric fraction Guinier plot ln 1.68×100 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Haloalkane dehalogenase variant DhaA115 - dimeric fraction Kratky plot 1.104 0 3 sRg
p(r)
Haloalkane dehalogenase variant DhaA115 - dimeric fraction pair distance distribution function Rg: 3.1 nm 0 Dmax: 8.9 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Haloalkane dehalogenase variant DhaA115 - dimeric fraction CORAL model

log I(s)
 s, nm-1
Haloalkane dehalogenase variant DhaA115 - dimeric fraction CORAL model

log I(s)
 s, nm-1
Haloalkane dehalogenase variant DhaA115 - dimeric fraction CORAL model

SAXS data from solutions of haloalkane dehalogenase variant DhaA115 in 50 mM potassium phosphate buffer (41 mM K₂HPO₄, 9mM KH₂PO₄), pH 7.5 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.2 and 8.7 mg/ml were measured at 20°C. Six successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Haloalkane dehalogenase variant DhaA115 - dimeric fraction (DhaA115 - dimeric)
Mol. type   Protein
Organism   Rhodococcus rhodochrous
Olig. state   Dimer
Mon. MW   34.4 kDa
Sequence   FASTA
 
PDB code   6TY7
 
PDB code   6XT8
 
PDB code   6XTC