Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst

Markova K, Chmelova K, Marques S, Carpentier P, Bednar D, Damborsky J, Marek M
Chemical Science 11(41):11162-11178 (2020)

doi: 10.1039/D0SC03367G
Submitted to SASBDB: 2020 Mar 2
Published in SASBDB:

SASDHP7 – Haloalkane dehalogenase variant DhaA115 - monomeric fraction

Haloalkane dehalogenase variant DhaA115 -monomeric fraction experimental SAS data
PDB model
Sample: Haloalkane dehalogenase variant DhaA115 -monomeric fraction monomer, 34 kDa Rhodococcus rhodochrous protein
Buffer: 50 mM potassium phosphate buffer (41 mM K₂HPO₄, 9mM KH₂PO₄), pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2019 Aug 22
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 41 nm3

SASDHQ7 – Haloalkane dehalogenase variant DhaA115 - dimeric fraction

Haloalkane dehalogenase variant DhaA115 - dimeric fraction experimental SAS data
CORAL model
Sample: Haloalkane dehalogenase variant DhaA115 - dimeric fraction dimer, 69 kDa Rhodococcus rhodochrous protein
Buffer: 50 mM potassium phosphate buffer (41 mM K₂HPO₄, 9mM KH₂PO₄), pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2019 Aug 22
RgGuinier 2.9 nm
Dmax 8.9 nm
VolumePorod 78 nm3