|
SANS data from solutions of the BinB/Cqm1 complex in 25 mM HEPES, pD 7.5, 25 mM NaCl, 100% D2O, were collected using a Ingenuously built detector at a wavelength of λ = 0.52 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 25°C. One 43200 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The BinB component of larvicidal binary toxin binds receptor Cqm1 with high affinity (KD, ~10 nM). The complex migrates as a single particle on Native-PAGE. From its molecular weight and stoichiometry, the complex consists of one molecule each of Cqm1 and BinB proteins. The SANS experiments were performed using a sample reconstituted in 100% D2O. CAUTION! The corresponding model fit to the data relates to the ab initio bead model displayed in this entry and not the atomistic high-resolution model.
|
| Binary larvicide subunit BinB
(binB)
|
| Mol. type |
|
Protein |
| Organism |
|
Lysinibacillus sphaericus |
| Olig. state |
|
Monomer |
| Mon. MW |
|
53.5 kDa |
| |
| UniProt |
|
P10565 (1-448) |
| Sequence |
|
FASTA |
| |
| Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT)
(Cqm1)
|
| Mol. type |
|
Protein |
| Organism |
|
Culex quinquefasciatus |
| Olig. state |
|
Monomer |
| Mon. MW |
|
64.7 kDa |
| |
| UniProt |
|
B0X223 (23-560) |
| Sequence |
|
FASTA |
| |
|
s, nm-1
