Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin.

Sharma M, Kumar A, Kumar V
J Membr Biol (2020 Jul 28)
PMID: 32725430
doi: 10.1007/s00232-020-00131-4
Submitted to SASBDB: 2020 Mar 20
Published in SASBDB:

SASDHR8 – Mosquito-larvicidal binary toxin receptor (Cqm1) protein

Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
PDB model
Sample: Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) dimer, 129 kDa Culex quinquefasciatus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
RgGuinier 4.0 nm
Dmax 9.2 nm
VolumePorod 97 nm3

SASDHS8 – Mosquito-larvicidal BinB protein

Binary larvicide subunit BinB experimental SAS data
PDB model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 48 nm3

SASDHT8 – Complex of mosquito-larvicidal BinB protein and the Mosquito-larvicidal binary toxin receptor (Cqm1)

Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
OTHER model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
RgGuinier 4.6 nm
Dmax 11.7 nm
VolumePorod 290 nm3

SASDHU8 – SANS study of the complex between mosquito-larvicidal BinB protein and the mosquito-larvicidal binary toxin receptor (Cqm1)

Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
OTHER model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 26
RgGuinier 3.7 nm
Dmax 12.9 nm
VolumePorod 174 nm3