Hyperstable De Novo Protein with a Dimeric Bisecting Topology.

Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R, ACS Synth Biol (2020) Europe PMC

SASDHX3 – Hyperstable de novo protein Super WA20 (SUWA)

Hyperstable de novo protein Super WA20
MWI(0) 24 kDa
MWexpected 25 kDa
log I(s) 1.90×10-3 1.90×10-4 1.90×10-5 1.90×10-6
Hyperstable de novo protein Super WA20 small angle scattering data  s, nm-1
ln I(s)
Hyperstable de novo protein Super WA20 Guinier plot ln 1.90×10-3 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Hyperstable de novo protein Super WA20 Kratky plot 1.104 0 3 sRg
p(r)
Hyperstable de novo protein Super WA20 pair distance distribution function Rg: 2.6 nm 0 Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Hyperstable de novo protein Super WA20 PDB model

log I(s)
 s, nm-1
Hyperstable de novo protein Super WA20 DAMMIN model

Synchrotron SAXS data from solutions of Hyperstable de novo protein Super WA20 (SUWA) in 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH 7.5 were collected on the BL-6A beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 1M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Hyperstable de novo protein Super WA20 (SUWA)
Mol. type   Protein
Organism   artificially designed de novo protein
Olig. state   Dimer
Mon. MW   12.6 kDa
Sequence   FASTA
 
PDB code   6KOS