Search

 
Advanced search  

10 hits found for Arai

SASDHW3 – Binary-patterned 4-helix bundle de novo protein WA20

De novo protein WA20 experimental SAS data
PDB model
Sample: De novo protein WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
...Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDHX3 – Hyperstable de novo protein Super WA20 (SUWA)

Hyperstable de novo protein Super WA20 experimental SAS data
PDB model
Sample: Hyperstable de novo protein Super WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
...Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDD46 – Artificially designed de novo protein esPN-Block (HL4) heterocomplex, e1s2 (HL4)

extender PN-Block (HL4)stopper PN-Block (WA20) experimental SAS data
DAMMIN model
Sample: extender PN-Block (HL4) monomer, 27 kDa de novo protein protein
stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19
Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
...Arai R
RgGuinier 3.6 nm
Dmax 15.0 nm

SASDD56 – Artificially designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4)

stopper PN-Block (WA20)extender PN-Block (FL4) experimental SAS data
DAMMIN model
Sample: stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein
extender PN-Block (FL4) monomer, 27 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19
Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
...Arai R
RgGuinier 3.3 nm
Dmax 12.0 nm

SASDKM8 – Rationally optimised WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

SASDKN8 – Rationally optimised WA20 (ROWA) tetramer

Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 4.1 nm
Dmax 18.1 nm
VolumePorod 60 nm3

SASDKP8 – Rationally optimised WA20 (ROWA) hexamer

Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer hexamer, 75 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 5.3 nm
Dmax 24.8 nm
VolumePorod 120 nm3

SASDKQ8 – Rationally optimised SUWA (ROSA) dimer

Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 2.5 nm
Dmax 10.6 nm
VolumePorod 30 nm3

SASDKR8 – Rationally optimised SUWA (ROSA) tetramer

Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 4.4 nm
Dmax 22.1 nm
VolumePorod 88 nm3

SASDDZ8 – Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil , 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Nov 11
Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces. Angew Chem Int Ed Engl (2018)
...Arai R, Miyamoto K
RgGuinier 9.4 nm
Dmax 23.0 nm