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50 hits found for Arai

SASDUC2 – TIP120 (designed based on Truncated Icosahedral Protein composed of 60-mer fusion proteins)

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsC-terminal split fragment of a single subunit of Truncated Isosahedral Protein composed of 60-mer fusion proteins experimental SAS data
DAMMIN model
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 360 kDa synthetic construct protein
C-terminal split fragment of a single subunit of Truncated Isosahedral Protein composed of 60-mer fusion proteins , 715 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage. Chem Commun (Camb) (2024)
...Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
RgGuinier 9.2 nm
Dmax 22.5 nm
VolumePorod 3480 nm3

SASDUD2 – mTIP120-Ba (metal-ion induced 120-mer complex of TIP120 mutant (K26E in C-terminal subunit) with Ba ions)

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsK26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins experimental SAS data
DAMMIN model
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 360 kDa synthetic construct protein
K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 857 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 4 mM BaCl2, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage. Chem Commun (Camb) (2024)
...Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
RgGuinier 9.4 nm
Dmax 22.4 nm
VolumePorod 3320 nm3

SASDUE2 – TIP120 mutant K26E in CoreC (C-terminal split fragment of a fusion protein that assembles to Truncated Icosahedral protein composed of TIP60 ) subunit with EDTA

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsK26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins experimental SAS data
N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins Kratky plot
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 360 kDa synthetic construct protein
K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 857 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 4 mM BaCl2, 32 mM EDTA, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage. Chem Commun (Camb) (2024)
...Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
RgGuinier 4.7 nm
Dmax 13.9 nm
VolumePorod 110 nm3

SASDPV2 – original TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil , 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
...Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 9.1 nm
Dmax 22.7 nm

SASDPW2 – mTIP60-Ba (metal-ion induced 60-mer complex of TIP60 (K67E) mutant with barium ions)

TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions)Barium ion experimental SAS data
DAMMIN model
Sample: TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) , 1066 kDa Artificial protein protein
Barium ion 0, 8 kDa
Buffer: 25 mM HEPES, 100 mM NaCl, 5% glycerol, 5 mM BaCl2, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
...Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 9.6 nm
Dmax 21.8 nm

SASDPX2 – TIP60 (K67E) mutant (dimer) with EDTA

TIP60 (K67E) mutant with EDTA experimental SAS data
DAMMIN model
Sample: TIP60 (K67E) mutant with EDTA dimer, 36 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
...Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 3.7 nm
Dmax 12.0 nm

SASDNC3 – Lectin nano-block dimer of WA20-SL-ACG

Lectin nano-block WA20-SL-ACG experimental SAS data
DAMMIN model
Sample: Lectin nano-block WA20-SL-ACG dimer, 60 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 20
Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans International Journal of Molecular Sciences 23(2):676 (2022)
...Arai R
RgGuinier 3.1 nm
Dmax 14.9 nm
VolumePorod 82 nm3

SASDND3 – Lectin nano-block tetramer of WA20-SL-ACG

Lectin nano-block WA20-SL-ACG experimental SAS data
CORAL model
Sample: Lectin nano-block WA20-SL-ACG tetramer, 121 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 20
Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans International Journal of Molecular Sciences 23(2):676 (2022)
...Arai R
RgGuinier 4.7 nm
Dmax 18.6 nm
VolumePorod 183 nm3

SASDNE3 – Lectin nano-block hexamer of WA20-SL-ACG

Lectin nano-block WA20-SL-ACG experimental SAS data
CORAL model
Sample: Lectin nano-block WA20-SL-ACG hexamer, 181 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Jun 20
Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans International Journal of Molecular Sciences 23(2):676 (2022)
...Arai R
RgGuinier 5.4 nm
Dmax 24.2 nm
VolumePorod 313 nm3

SASDNF3 – Lectin nano-block dimer of WA20-H-ACG

Lectin nano-block WA20-H-ACG experimental SAS data
DAMMIN model
Sample: Lectin nano-block WA20-H-ACG dimer, 59 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Oct 31
Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans International Journal of Molecular Sciences 23(2):676 (2022)
...Arai R
RgGuinier 3.9 nm
Dmax 18.1 nm
VolumePorod 85 nm3

SASDNG3 – Lectin nano-block dimer of WA20-ΔN3ACG

Lectin nano-block WA20-ΔN3ACG experimental SAS data
DAMMIN model
Sample: Lectin nano-block WA20-ΔN3ACG dimer, 58 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Oct 31
Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans International Journal of Molecular Sciences 23(2):676 (2022)
...Arai R
RgGuinier 4.1 nm
Dmax 21.6 nm
VolumePorod 87 nm3

SASDHW3 – Binary-patterned 4-helix bundle de novo protein WA20

De novo protein WA20 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: De novo protein WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
...Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDHX3 – Hyperstable de novo protein Super WA20 (SUWA)

Hyperstable de novo protein Super WA20 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Hyperstable de novo protein Super WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
...Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDD46 – Artificially designed de novo protein esPN-Block (HL4) heterocomplex, e1s2 (HL4)

extender PN-Block (HL4)stopper PN-Block (WA20) experimental SAS data
DAMMIN model
Sample: extender PN-Block (HL4) monomer, 27 kDa de novo protein protein
stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19
Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
...Arai R
RgGuinier 3.6 nm
Dmax 15.0 nm

SASDD56 – Artificially designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4)

stopper PN-Block (WA20)extender PN-Block (FL4) experimental SAS data
DAMMIN model
Sample: stopper PN-Block (WA20) dimer, 25 kDa de novo protein protein
extender PN-Block (FL4) monomer, 27 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2014 Dec 19
Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks. ACS Synth Biol 7(5):1381-1394 (2018)
...Arai R
RgGuinier 3.3 nm
Dmax 12.0 nm

SASDLF7 – The Fe–S cluster assembly 1 homolog of pigeon (clISCA1) without the magnetic circuit

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Pigeon iron-sulfur cluster assembly 1 homolog (clISCA1) 29.3 mg/ml
Shigeki Arai
RgGuinier 4.5 nm
Dmax 15.8 nm
VolumePorod 130 nm3

SASDLT7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 1min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 4.3 nm
Dmax 15.8 nm

SASDLU7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 2min

Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 4.4 nm

SASDLV7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 3min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 4.6 nm

SASDLW7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 4min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 4.5 nm

SASDLX7 – The Fe–S cluster assembly 1 homolog of pigeon (29.3 mg/ml), Total X-ray exposure time : 5min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 4.4 nm

SASDLY7 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 1min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 2.7 nm

SASDLZ7 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 2min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 2.8 nm

SASDL28 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 3min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 2.7 nm

SASDL38 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 4min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 2.7 nm

SASDL48 – The Fe–S cluster assembly 1 homolog of pigeon (6.9 mg/ml), Total X-ray exposure time : 5min

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8
Radiation resistivity of clISCA1
Shigeki Arai
RgGuinier 2.7 nm

SASDQ88 – recombinant p123 expressed protein (Gli123)

p123 expressed protein experimental SAS data
DAMMIN model
Sample: p123 expressed protein dimer, 248 kDa Mycoplasma mobile (strain … protein
Buffer: 100 mM ammonium acetate, pH: 6.8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Oct 25
Structure and Function of Gli123 Involved in Mycoplasma mobile Gliding. J Bacteriol :e0034022 (2023)
...Arai M, Miyata M
RgGuinier 8.2 nm
Dmax 31.6 nm
VolumePorod 436 nm3

SASDRJ8 – A de novo enzyme, ferric enterobactin esterase Syn-F4

De novo enterobactin esterase Syn-F4 (K4T) experimental SAS data
DAMMIN model
Sample: De novo enterobactin esterase Syn-F4 (K4T) dimer, 25 kDa synthetic construct protein
Buffer: 25 mM MES, 100 mM NaCl, 10% glycerol, 200 mM Arg-HCl, pH: 6.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Feb 11
Crystal structure and activity of a de novo enzyme, ferric enterobactin esterase Syn-F4. Proc Natl Acad Sci U S A 120(38):e2218281120 (2023)
...Arai R
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 40 nm3

SASDKM8 – Rationally optimised WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

SASDKN8 – Rationally optimised WA20 (ROWA) tetramer

Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 4.1 nm
Dmax 18.1 nm
VolumePorod 60 nm3

SASDKP8 – Rationally optimised WA20 (ROWA) hexamer

Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer hexamer, 75 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 5.3 nm
Dmax 24.8 nm
VolumePorod 120 nm3

SASDKQ8 – Rationally optimised SUWA (ROSA) dimer

Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 2.5 nm
Dmax 10.6 nm
VolumePorod 30 nm3

SASDKR8 – Rationally optimised SUWA (ROSA) tetramer

Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
...Arai R
RgGuinier 4.4 nm
Dmax 22.1 nm
VolumePorod 88 nm3

SASDDZ8 – Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil , 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Nov 11
Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces. Angew Chem Int Ed Engl (2018)
...Arai R, Miyamoto K
RgGuinier 9.4 nm
Dmax 23.0 nm

SASDLD8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.270)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.2 nm

SASDLE8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.280)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.2 nm

SASDLF8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.290)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.1 nm

SASDLG8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.300)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.0 nm

SASDLH8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.310)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 1.9 nm

SASDLJ8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.320)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.0 nm

SASDLK8 – The Fe–S cluster assembly 1 homolog of pigeon (Elution No.330)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
ROSETTA model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Feb 25
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 2.0 nm

SASDL58 – The Fe–S cluster assembly 1 homolog of pigeon before applying the external magnetic field (0 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 4.7 nm
Dmax 15.8 nm

SASDL68 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 5 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.0 nm
Dmax 16.2 nm

SASDL78 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 10 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 4.9 nm
Dmax 16.5 nm

SASDL88 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 30 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.1 nm
Dmax 17.2 nm

SASDL98 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 60 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.5 nm
Dmax 19.1 nm

SASDLA8 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.5 : 5 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.7 nm
Dmax 18.9 nm

SASDLL8 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.5 : 10 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.7 nm
Dmax 18.9 nm

SASDLB8 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.5 : 30 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.4 nm
Dmax 19.0 nm

SASDLC8 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.5 : 60 min)

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial , 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 5.7 nm
Dmax 21.2 nm