data from solutions of
Escherichia coli RnlA (mRNA endoribonuclease toxin LS)
20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH 8
BM29 beam line
at the ESRF storage ring
using a Pilatus 1M detector
at a sample-detector distance of 2.8 m and
at a wavelength of λ = 0.09919 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.7 and 2.4 mg/ml were measured
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.