Quaternary structure changes control ribonuclease activity of Escherichia coli RnlA toxin

Gabriela Garcia-Rodriguez.

SASDHY7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged
MWexperimental 78 kDa
MWexpected 84 kDa
VPorod 133 nm3
log I(s) 8.69×101 8.69×100 8.69×10-1 8.69×10-2
mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged small angle scattering data  s, nm-1
ln I(s)
mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Guinier plot ln 8.70×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot 1.104 0 3 sRg
p(r)
mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged pair distance distribution function Rg: 4.1 nm 0 Dmax: 20.1 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant in 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 13 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW402.5-4F column at 20°C. 900 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged (R255A RnlA)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Dimer
Mon. MW   41.9 kDa
 
UniProt   P52129 (2-357)
Sequence   FASTA