A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism.

Büttner H, Perbandt M, Kohler T, Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H, mBio 11(5) (2020) Europe PMC

SASDJ92 – S. epidermidis extracellular matrix binding protein (Embp) F-repeats

Extracellular matrix binding protein F-repeats
MWI(0) 82 kDa
MWexpected 70 kDa
log I(s) 9.06×101 9.06×100 9.06×10-1 9.06×10-2
Extracellular matrix binding protein F-repeats small angle scattering data  s, nm-1
ln I(s)
Extracellular matrix binding protein F-repeats Guinier plot ln 9.07×101 Rg: 7.0 nm 0 (7.0 nm)-2 s2
(sRg)2I(s)/I(0)
Extracellular matrix binding protein F-repeats Kratky plot 1.104 0 √3 sRg
p(r)
Extracellular matrix binding protein F-repeats pair distance distribution function Rg: 7.7 nm 0 Dmax: 28 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Extracellular matrix binding protein F-repeats SASREF model
log I(s)
 s, nm-1
Extracellular matrix binding protein F-repeats GASBOR model
Synchrotron SAXS data from solutions of S. epidermidis extracellular matrix binding protein (Embp) F-repeats in 50 mM MES, 150 mM NaCl, pH 6 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.10 mg/ml was measured at 10.1°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Extracellular matrix binding protein F-repeats (rEmbp2588)
Mol. type   Protein
Organism   Staphylococcus epidermidis
Olig. state   Other
Mon. MW   70.4 kDa
 
UniProt   Q5HPA2
Sequence   FASTA
 
PDB code   6GV8