A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism.

Büttner H, Perbandt M, Kohler T, Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H
mBio 11(5) (2020 Oct 20)
PMID: 33082256
doi: 10.1128/mBio.01612-20 		Submitted to SASBDB: 2020 Apr 16
Published in SASBDB:

SASDJ92 – S. epidermidis extracellular matrix binding protein (Embp) F-repeats

Extracellular matrix binding protein F-repeats experimental SAS data
SASREF model
Sample: Extracellular matrix binding protein F-repeats, 70 kDa Staphylococcus epidermidis protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 15
RgGuinier 7.0 nm
Dmax 28.0 nm

SASDJA2 – S. epidermidis extracellular matrix binding protein (Embp) FG-repeats

Extracellular matrix binding protein FG-repeats experimental SAS data
EOM/RANCH model
Sample: Extracellular matrix binding protein FG-repeats, 85 kDa Staphylococcus epidermidis protein
Buffer: PBS, 136.5 mM NaCl, 2.65 mM KCl, 8.3 mM Na2HPO4, 2.65 mM KH2PO4, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14
RgGuinier 10.7 nm
Dmax 40.0 nm