data from solutions of
Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) full length
25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH 8
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample
at 5 mg/ml was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.