Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments.

Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S
Structure (2020 Aug 15)
PMID: 32846144
doi: 10.1016/j.str.2020.08.001
Submitted to SASBDB: 2020 Jun 5
Published in SASBDB:

SASDJA4 – Endonuclease VIII from E. coli (size exclusion chromatography SAXS)

Endonuclease 8 experimental SAS data
PDB model
Sample: Endonuclease 8 monomer, 31 kDa Escherichia coli protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 45 nm3

SASDJB4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) full length

Nei like DNA glycosylase 2 experimental SAS data
Nei like DNA glycosylase 2 Kratky plot
Sample: Nei like DNA glycosylase 2 monomer, 39 kDa Monodelphis domestica protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 58 nm3

SASDJC4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133)

Nei like DNA glycosylase 2 (Δ67-133) experimental SAS data
PDB model
Sample: Nei like DNA glycosylase 2 (Δ67-133) monomer, 33 kDa Monodelphis domestica protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 47 nm3