Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.

Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V, Nucleic Acids Res 45(6):3547-3558 (2017) Europe PMC

SASDJC2 – Restriction endonuclease R.AgeI complex with cognate DNA

Type-2 restriction enzyme AgeI
Cognate DNA oligoduplex with 5'-T overhang
MWI(0) 65 kDa
MWexpected 69 kDa
VPorod 69 nm3
log I(s) 6.40×10-2 6.40×10-3 6.40×10-4 6.40×10-5
Type-2 restriction enzyme AgeI Cognate DNA oligoduplex with 5'-T overhang small angle scattering data  s, nm-1
ln I(s)
Type-2 restriction enzyme AgeI Cognate DNA oligoduplex with 5'-T overhang Guinier plot ln 6.40×10-2 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Type-2 restriction enzyme AgeI Cognate DNA oligoduplex with 5'-T overhang Kratky plot 1.104 0 3 sRg
p(r)
Type-2 restriction enzyme AgeI Cognate DNA oligoduplex with 5'-T overhang pair distance distribution function Rg: 2.4 nm 0 Dmax: 7.4 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Type-2 restriction enzyme AgeI Cognate DNA oligoduplex with 5'-T overhang PDB model

log I(s)
 s, nm-1
Type-2 restriction enzyme AgeI Cognate DNA oligoduplex with 5'-T overhang DAMMIN model

Synchrotron SAXS data from solutions of the R.AgeI complex with cognate DNA in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂ were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.80 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Type-2 restriction enzyme AgeI (R.AgeI)
Mol. type   Protein
Organism   Thalassobius gelatinovorus
Olig. state   Dimer
Mon. MW   30.5 kDa
 
UniProt   Q9KHV6 (1-278)
Sequence   FASTA
 
Cognate DNA oligoduplex with 5'-T overhang (SP13)
Mol. type   DNA
Olig. state   Dimer
Mon. MW   4.0 kDa
Sequence   FASTA
 
PDB code   5DWB