Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.

Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V
Nucleic Acids Res 45(6):3547-3558 (2017 Apr 7)
PMID: 28039325
doi: 10.1093/nar/gkw1310
Submitted to SASBDB: 2020 Apr 14
Published in SASBDB:

SASDJB2 – Restriction endonuclease R.AgeI in apo form, monomeric

Type-2 restriction enzyme AgeI experimental SAS data
DAMMIN model
Sample: Type-2 restriction enzyme AgeI monomer, 31 kDa Thalassobius gelatinovorus protein
Buffer: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaClâ‚‚, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14
RgGuinier 2.2 nm
Dmax 6.7 nm
VolumePorod 53 nm3

SASDJC2 – Restriction endonuclease R.AgeI complex with cognate DNA

Type-2 restriction enzyme AgeICognate DNA oligoduplex with 5'-T overhang experimental SAS data
PDB model
Sample: Type-2 restriction enzyme AgeI dimer, 61 kDa Thalassobius gelatinovorus protein
Cognate DNA oligoduplex with 5'-T overhang dimer, 8 kDa DNA
Buffer: 10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaClâ‚‚, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14
RgGuinier 2.4 nm
Dmax 7.4 nm
VolumePorod 69 nm3