Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments.

Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S, Structure (2020) Europe PMC

SASDJC4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133)

Nei like DNA glycosylase 2 (Δ67-133)
MWI(0) 30 kDa
MWexpected 33 kDa
VPorod 47 nm3
log I(s) 3.53×102 3.53×101 3.53×100 3.53×10-1
Nei like DNA glycosylase 2 (Δ67-133) small angle scattering data  s, nm-1
ln I(s)
Nei like DNA glycosylase 2 (Δ67-133) Guinier plot ln 3.53×102 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Nei like DNA glycosylase 2 (Δ67-133) Kratky plot 1.104 0 3 sRg
p(r)
Nei like DNA glycosylase 2 (Δ67-133) pair distance distribution function Rg: 2.5 nm 0 Dmax: 7.8 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Nei like DNA glycosylase 2 (Δ67-133) PDB model

log I(s)
 s, nm-1
Nei like DNA glycosylase 2 (Δ67-133) PDB model

Synchrotron SAXS data from solutions of Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133) in 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data collected with in-line SEC

Nei like DNA glycosylase 2 (Δ67-133) (MdoNEIL2 cut)
Mol. type   Protein
Organism   Monodelphis domestica
Olig. state   Monomer
Mon. MW   32.7 kDa
 
UniProt   F7AMK3
Sequence   FASTA
 
PDB code   6VJI
 
PDB code   6VJI