Synchrotron SAXS data from solutions of the KASH5/SUN1 6:6 complex in 20 mM Tris pH 8.0, 150 mM KCl, were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.095 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 12 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 25 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
SUN domain-containing protein 1
(SUN1)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Hexamer |
Mon. MW |
|
22.5 kDa |
|
UniProt |
|
O94901
(616-812)
|
Sequence |
|
FASTA |
|
Protein KASH5
(KASH5)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Hexamer |
Mon. MW |
|
3.3 kDa |
|
UniProt |
|
Q8N6L0
(542-562)
|
Sequence |
|
FASTA |
|
PDB code |
|
6R2I |
|
PDB code |
|
6R2I |
|
|