A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.

Gurusaran M, Davies OR
Elife 10 (2021 Jan 4)

PMID: 33393904 doi: 10.7554/eLife.60175

Submitted to SASBDB: 2020 Jun 29 Published in SASBDB: 2021 Jan 5

SASDJF5 – SUN domain of SUN1 harbouring mutation I673E - monomer Sample: SUN domain-containing protein 1, I673E monomer, 23 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jul 29 RgGuinier 2.1 nm Dmax 8.2 nm VolumePorod 42 nm3

SASDJC5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-4 - SUN1-KASH4 6:6 complex Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein Nesprin-4 hexamer, 20 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 21 RgGuinier 4.0 nm Dmax 13.5 nm VolumePorod 240 nm3

SASDJD5 – LINC complex between the SUN domain of SUN1 and KASH domain of KASH5 - SUN1-KASH5 6:6 complex Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein Protein KASH5 hexamer, 20 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17 RgGuinier 3.8 nm Dmax 13.5 nm VolumePorod 244 nm3

SASDJE5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-1 - SUN1-KASH1 6:6 complex Sample: SUN domain-containing protein 1 hexamer, 135 kDa Homo sapiens protein Nesprin-1 hexamer, 22 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 17 RgGuinier 3.9 nm Dmax 13.0 nm VolumePorod 254 nm3