Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.

Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K, Sci Adv 7(22) (2021) Europe PMC

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)
MWexperimental 34 kDa
MWexpected 22 kDa
VPorod 66 nm3
log I(s) 2.20×101 2.20×100 2.20×10-1 2.20×10-2
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) small angle scattering data  s, nm-1
ln I(s)
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) Guinier plot ln 2.20×101 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) Kratky plot 1.104 0 3 sRg
p(r)
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) pair distance distribution function Rg: 4.1 nm 0 Dmax: 17.3 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram Rg, nm
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) EOM/RANCH model

Synchrotron SAXS data from solutions of Δ91-FATZ-1 in 50 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 4 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superose 6 Increase 10/300 column at 20°C. 44 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) (Δ91-FATZ-1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.5 kDa
 
UniProt   Q9NP98 (92-299)
Sequence   FASTA