Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.

Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
Sci Adv 7(22) (2021 May)
PMID: 34049882
doi: 10.1126/sciadv.abg7653
Submitted to SASBDB: 2020 Aug 12
Published in SASBDB:

SASDJJ6 – Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1)

N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data
Sarcomeric intrinsically disordered protein FATZ-1 (N-FATZ-1) Rg histogram
Sample: N-ter construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 20 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 29
RgGuinier 3.5 nm
Dmax 14.1 nm
VolumePorod 46 nm3

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data
Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram
Sample: Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 18
RgGuinier 3.9 nm
Dmax 17.3 nm
VolumePorod 66 nm3

SASDJL6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2)

Rod domain of α-actinin-2 experimental SAS data
PDB model
Sample: Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5
RgGuinier 6.7 nm
Dmax 27.2 nm
VolumePorod 214 nm3

SASDJM6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd)

Half dimer of α-actinin-2 experimental SAS data
Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd) Rg histogram
Sample: Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 18
RgGuinier 5.3 nm
Dmax 22.0 nm
VolumePorod 172 nm3

SASDJN6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1)

Rod domain of α-actinin-2Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data
Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) Rg histogram
Sample: Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 18
RgGuinier 7.6 nm
Dmax 28.4 nm
VolumePorod 371 nm3

SASDJP6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)Half dimer of α-actinin-2 experimental SAS data
Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1) Rg histogram
Sample: Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 18
RgGuinier 5.8 nm
Dmax 22.5 nm
VolumePorod 242 nm3