Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses.

Zhang CM, Imoto Y, Hikima T, Inoue T, J Struct Biol X 5:100045 (2021) Europe PMC

SASDJN5 – Isolated translocation domain of tetanus nerotoxin (TeNT/iHn)

Tetanus toxin (C467S)
MWexperimental 44 kDa
MWexpected 46 kDa
VPorod 54 nm3
log I(s) 1.15×10-2 1.15×10-3 1.15×10-4 1.15×10-5
Tetanus toxin (C467S) small angle scattering data  s, nm-1
ln I(s)
Tetanus toxin (C467S) Guinier plot ln 1.16×10-2 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Tetanus toxin (C467S) Kratky plot 1.104 0 3 sRg
p(r)
Tetanus toxin (C467S) pair distance distribution function Rg: 3.2 nm 0 Dmax: 12.1 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Isolated translocation domain of tetanus nerotoxin (TeNT/iHn) Rg histogram Rg, nm
Tetanus toxin (C467S) EOM/RANCH model
Tetanus toxin (C467S) EOM/RANCH model
Tetanus toxin (C467S) EOM/RANCH model

log I(s)
 s, nm-1
Tetanus toxin (C467S) DAMMIN model

log I(s)
 s, nm-1
Tetanus toxin (C467S) PDB model

Synchrotron SAXS data from solutions of the isolated translocation domain of the tetanus nerotoxin (TeNT/iHn) in 10 mM HEPES 100 mM NaCl, pH 7.4 were collected on the BL45XU beam line at SPring-8 (Hyōgo Prefecture, Japan) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 5 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 1200 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tetanus toxin (C467S) (TeNT/iHn)
Mol. type   Protein
Organism   Clostridium tetani
Olig. state   Monomer
Mon. MW   46.5 kDa
 
UniProt   P04958 (459-863)
Sequence   FASTA
 
PDB code   7BY5
 
PDB code   7BY5