Synchrotron SAXS data from solutions of the beltless isolated translocation domain of the tetanus nerotoxin (TeNT/blHn) in 10 mM HEPES 100 mM NaCl, pH 7.4 were collected on the BL45XU beam line at SPring-8 (Hyōgo Prefecture, Japan) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 5 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 800 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
Tetanus toxin
(TeNT/blHn)
|
Mol. type |
|
Protein |
Organism |
|
Clostridium tetani |
Olig. state |
|
Monomer |
Mon. MW |
|
34.6 kDa |
|
UniProt |
|
P04958
(564-864)
|
Sequence |
|
FASTA |
|
PDB code |
|
7BXX |
|
|