Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations

Nguyen G Sutinen A, Raasakka A, Muruganandam G, Loris R, Kursula P, Frontiers in Molecular Biosciences 7 (2021) DOI

SASDJR8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358
MWexperimental 76 kDa
MWexpected 68 kDa
VPorod 111 nm3
log I(s) 5.75×103 5.75×102 5.75×101 5.75×100
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 small angle scattering data  s, nm-1
ln I(s)
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 Guinier plot ln 5.76×103 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 Kratky plot 1.104 0 √3 sRg
p(r)
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 pair distance distribution function Rg: 31.2 nm 0 Dmax: 101.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 GASBOR model
Synchrotron SAXS data from solutions of dimeric human GDAP1∆295-358 in 25 mM HEPES, 300 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 15°C. 3000 successive 0.045 second frames were collected through the SEC elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the sample elution peak frames to generate the final SAXS profile.

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 (GDAP1∆295-358)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   34.2 kDa
 
UniProt   Q8TB36 (1-294)
Sequence   FASTA