Molecular model of a sensor of two-component signaling system

Yury Ryzhykau.

SASDK37 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonis
Sensory rhodopsin II transducer from Natronomonas pharaonis
MWexperimental 175 kDa
MWexpected 169 kDa
log I(s) 1.07×100 1.07×10-1 1.07×10-2 1.07×10-3
Sensory rhodopsin II from Natronbacterium pharaonis Sensory rhodopsin II transducer from Natronomonas pharaonis small angle scattering data  s, nm-1
ln I(s)
Sensory rhodopsin II from Natronbacterium pharaonis Sensory rhodopsin II transducer from Natronomonas pharaonis Guinier plot ln 1.07×100 Rg: 7.1 nm 0 (7.1 nm)-2 s2
(sRg)2I(s)/I(0)
Sensory rhodopsin II from Natronbacterium pharaonis Sensory rhodopsin II transducer from Natronomonas pharaonis Kratky plot 1.104 0 3 sRg
p(r)
Sensory rhodopsin II from Natronbacterium pharaonis Sensory rhodopsin II transducer from Natronomonas pharaonis pair distance distribution function Rg: 8.0 nm 0 Dmax: 35 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Sensory rhodopsin II from Natronbacterium pharaonis Sensory rhodopsin II transducer from Natronomonas pharaonis MEMPROT model

SANS data from solutions of the sensory rhodopsin II / transducer complex in 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pD 8 were collected using the YuMO (IBR-2) instrument (FLNP, JINR; Dubna, Russian Federation) equipped with a He3-fulfilled, 8 independent wire detector. One solute concentration of 0.51 mg/ml was measured at 20°C. SANS measurements were performed with a two-detector system (sample-detector distances of 4.5 m and 12.97 m). The wavelengths used are from 0.05 to 0.8 nm, the contributions of which are separated using time-of-flight technology.

The protein of study is a non-fused complex of sensory rhodopsin II (NpSRII, UniProt ID P42196) with its cognate transducer (NpHtrII, UniProt ID P42259) from Nartonomonas pharaonis. The NpSRII/NpHtrII complex in 0.05% (w/v) n-Dodecyl β-D-maltoside (DDM) detergent forms dimers at 150 mM NaCl.

Sensory rhodopsin II from Natronbacterium pharaonis (NpSRII)
Mol. type   Protein
Organism   Natronomonas pharaonis
Olig. state   Dimer
Mon. MW   26.7 kDa
 
UniProt   P42196 (1-239)
Sequence   FASTA
 
Sensory rhodopsin II transducer from Natronomonas pharaonis (NpHtrII)
Mol. type   Protein
Organism   Natronomonas pharaonis
Olig. state   Dimer
Mon. MW   57.8 kDa
 
UniProt   P42259 (3-534)
Sequence   FASTA