Molecular model of a sensor of two-component signaling system

Yury Ryzhykau Submitted to SASBDB: 2021 Jan 4
Published in SASBDB:

SASDKZ6 – SAXS data for the complex of the sensory rhodopsin II with its cognate truncated transducer in detergent at 0.15 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisTruncated sensory rhodopsin II transducer containing transmembrane and HAMP1 domains experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Truncated sensory rhodopsin II transducer containing transmembrane and HAMP1 domains dimer, 30 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 Na/K-Pi, 1 mM EDTA, 0.05 % DDM, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 13
RgGuinier 4.8 nm
Dmax 14.0 nm

SASDK27 – SAXS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 9
RgGuinier 9.0 nm
Dmax 38.9 nm

SASDK37 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO, IBR-2, FLNP, JINR on 2019 Jan 25
RgGuinier 7.1 nm
Dmax 35.0 nm

SASDK47 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 1.4 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 1400 mM NaCl, 49.4 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO, IBR-2, FLNP, JINR on 2019 Feb 10
RgGuinier 9.9 nm
Dmax 36.5 nm

SASDK57 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 2.8 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 2800 mM NaCl, 76.6 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO, IBR-2, FLNP, JINR on 2019 Feb 10
RgGuinier 9.3 nm
Dmax 37.5 nm

SASDK67 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 4.0 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 4000 mM NaCl, 100 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO, IBR-2, FLNP, JINR on 2019 Jan 25
RgGuinier 8.9 nm
Dmax 39.0 nm