E3 ubiquitin/ISG15 ligase TRIM25

Kevin Haubrich.

SASDK78 – E3 ubiquitin/ISG15 ligase TRIM25, apo form (TRIM25 apo)

E3 ubiquitin/ISG15 ligase TRIM25
MWexperimental 105 kDa
MWexpected 100 kDa
log I(s) 1.50×102 1.50×101 1.50×100 1.50×10-1
E3 ubiquitin/ISG15 ligase TRIM25 small angle scattering data  s, nm-1
ln I(s)
E3 ubiquitin/ISG15 ligase TRIM25 Guinier plot ln 1.51×102 Rg: 6.8 nm 0 (6.8 nm)-2 s2
(sRg)2I(s)/I(0)
E3 ubiquitin/ISG15 ligase TRIM25 Kratky plot 1.104 0 3 sRg
p(r)
E3 ubiquitin/ISG15 ligase TRIM25 pair distance distribution function Rg: 7.2 nm 0 Dmax: 30.2 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of TRIM25 apo in 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH 6.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.122 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 6 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   50.1 kDa
 
UniProt   Q14258 (189-630)
Sequence   FASTA