E3 ubiquitin/ISG15 ligase TRIM25

Kevin Haubrich Submitted to SASBDB: 2021 Jan 28
Published in SASBDB:

SASDK78 – E3 ubiquitin/ISG15 ligase TRIM25, apo form (TRIM25 apo)

E3 ubiquitin/ISG15 ligase TRIM25 experimental SAS data
E3 ubiquitin/ISG15 ligase TRIM25 Kratky plot
Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein
Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 19
RgGuinier 6.8 nm
Dmax 30.2 nm

SASDK88 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7)

E3 ubiquitin/ISG15 ligase TRIM25pre-let-7-a-1@1 experimental SAS data
E3 ubiquitin/ISG15 ligase TRIM25 pre-let-7-a-1@1 Kratky plot
Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein
Pre-let-7-a-1@1 monomer, 9 kDa synthetic construct RNA
Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 14
RgGuinier 5.7 nm
Dmax 19.8 nm

SASDK98 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7): SEC-SAXS

E3 ubiquitin/ISG15 ligase TRIM25pre-let-7-a-1@1 experimental SAS data
E3 ubiquitin/ISG15 ligase TRIM25 pre-let-7-a-1@1 Kratky plot
Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein
Pre-let-7-a-1@1 monomer, 9 kDa synthetic construct RNA
Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 4
RgGuinier 5.7 nm
Dmax 23.0 nm

SASDKA8 – E3 ubiquitin/ISG15 ligase TRIM25 bound to lnczc3h7a_304-326 RNA (TRIM25/lnczc3h7a)

E3 ubiquitin/ISG15 ligase TRIM25lnczc3h7a_304-326 experimental SAS data
E3 ubiquitin/ISG15 ligase TRIM25 lnczc3h7a_304-326 Kratky plot
Sample: E3 ubiquitin/ISG15 ligase TRIM25 dimer, 100 kDa Homo sapiens protein
Lnczc3h7a_304-326 monomer, 8 kDa Homo sapiens RNA
Buffer: 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 12
RgGuinier 5.8 nm
Dmax 16.6 nm