Synchrotron SAXS data from solutions of TRIM25/pre-let-7 in 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH 6.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.125 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 100 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The concentration estimate quoted for this entry is likely inaccurate due to the uncertainty of the complex's molar extinction coefficient (protein plus RNA).