Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane.

Park SH, Han J, Jeong BC, Song JH, Jang SH, Jeong H, Kim BH, Ko YG, Park ZY, Lee KE, Hyun J, Song HK, Nat Struct Mol Biol (2023) Europe PMC

SASDK96 – tripartite motif-containing protein 72 (TRIM72) lacking the zinc finger RING domain (ΔRING)

Tripartite motif-containing protein 72
MWexperimental 98 kDa
MWexpected 88 kDa
VPorod 142 nm3
log I(s) 2.08×10-3 2.08×10-4 2.08×10-5 2.08×10-6
Tripartite motif-containing protein 72 small angle scattering data  s, nm-1
ln I(s)
Tripartite motif-containing protein 72 Guinier plot ln 2.09×10-3 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
Tripartite motif-containing protein 72 Kratky plot 1.104 0 √3 sRg
p(r)
Tripartite motif-containing protein 72 pair distance distribution function Rg: 5.2 nm 0 Dmax: 18.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Tripartite motif-containing protein 72 OTHER model
log I(s)
 s, nm-1
Tripartite motif-containing protein 72 DAMMIN model
log I(s)
 s, nm-1
Tripartite motif-containing protein 72 DAMMIN model
Synchrotron SAXS data from solutions of TRIM72-ΔRING) in 25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM TCEP, 1 mM DTT, were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 240.00 μl sample at 13 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 190 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tripartite motif-containing protein 72 (TRIM72)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   44.1 kDa
 
UniProt   Q1XH17 (79-470)
Sequence   FASTA
 
PDB ID   7DEW